Group of “Molecular-Cellular Ageing and Carcinogenesis (MCAC)”

Publications - Patents




Tsakiri Å.Í. & Trougakos I.P. (2014). The Amazing Ubiquitin-Proteasome System: Structural Components and Implication in Aging. Int Rev Cell Mol Biol. (in press)

Niforou K., Cheimonidou C. & Trougakos I.P. (2014). Molecular chaperones and proteostasis regulation during redox imbalance. Redox Biol. 2, 323-332.



Argyropoulou A., Aligiannis N., Trougakos I.P., & Skaltsounis A.L. (2013). Natural compounds with anti-ageing activity. Nat. Prod. Rep. 30, 1412-1437.

Ioannou K., Derhovanessian E., Tsakiri E., Samara P., Kalbacher H., Voelter W., Trougakos I.P., Pawelec G. & Tsitsilonis O.E. (2013). Prothymosin alpha and a prothymosin alpha-derived peptide enhance TH1-type immune responses against defined HER-2/neu epitopes. BMC Immunol. 14, 43.

Tsakiri E.N., Iliaki K.K., Höhn A., Grimm S., Papassideri I.S., Grune T. & Trougakos I.P. (2013). Diet-derived Advanced Glycation End products or Lipofuscin disrupt proteostasis and reduce lifespan in Drosophila melanogaster. Free Rad. Biol. Med. 65, 1155-1163.

Evangelou K., Bartkova J., Kotsinas A., Pateras I.S., Liontos M., Velimezi G., Kosar M., Liloglou T., Trougakos I., Dyrskjot L., Andersen C.L., Papaioannou M., Drosos Y., Papafotiou G., Hodny Z., Sosa-Pineda B., Wu X-R., Klinakis A., Ørntoft T., Lukas J., Bartek J. & Gorgoulis V. (2013). The DNA damage checkpoint precedes activation of ARF in response to escalating oncogenic stress during tumourigenesis. Cell Death Differ. 20, 1485-1497. EDITORIAL

Tsakiri E.N., Sykiotis G.P., Papassideri I.S., Terpos E., Dimopoulos M.A., Gorgoulis V.G., Bohmann D., & Trougakos I.P. (2013) Proteasome dysfunction in Drosophila signals to an Nrf2-dependent regulatory circuit aiming to restore proteostasis and prevent premature aging. Aging Cell 12, 802-813. RESEARCH HIGHLIGHT

Nedić O., Rattan S.I., Grune T. & Trougakos I.P. (2013). Molecular effects of advanced glycation end products (AGEs) on cell signalling pathways, ageing and pathophysiology. Free Radic Res. 47 Suppl 1, 28-38.

Trougakos I.P. (2013). The Molecular Chaperone Apolipoprotein J/Clusterin as a Sensor of Oxidative Stress: Implications in Therapeutic Approaches - A Mini-Review. Gerontology 59, 514-523.

Trougakos I.P., Sesti F., Tsakiri E. & Gorgoulis V.G. (2013). Non-enzymatic post-translational protein modifications and proteostasis network deregulation in carcinogenesis. J. Proteomics 92, 274-298.

Tsakiri E.N., Sykiotis G.P., Papassideri I.S., Gorgoulis V.G., Bohmann D., & Trougakos I.P. (2013). Differential regulation of proteasome functionality in reproductive vs. somatic tissues of Drosophila during aging or oxidative stress. FASEB J. 27, 2407-2420.

Georgakopoulou E.A., Tsimaratou K., Evangelou K., Fernandez Marcos P.J., Zoumpourlis V., Trougakos I.P., Kletsas D., Bartek J., Serrano M., & Gorgoulis VG. (2013). Specific lipofuscin staining as a novel biomarker to detect replicative and stress-induced senescence. A method applicable in cryo-preserved and archival tissues. Aging (Albany NY) 5, 37-50.


Sesti F., Tsitsilonis O.E., Kotsinas A. & Trougakos I.P. (2012). Oxidative stress-mediated biomolecular damage and inflammation in tumorigenesis. In Vivo 26, 395-402.


Sideridou M., Zakopoulou R., Evangelou K., Liontos M., Kotsinas A., Rampakakis E., Gagos S., Kahata K., Grabusic K., Gkouskou K., Trougakos I.P., Kolettas E., Georgakilas A.G., Volarevic S., Eliopoulos A.G., Zannis-Hadjopoulos M., Moustakas A., & Gorgoulis V.G. (2011). Cdc6 expression represses E-cadherin transcription and activates adjacent replication origins. J. Cell Biol. 195, 1123-1140. RESEARCH HIGHLIGHT

Antonelou M.H., Kriebardis A.G., Stamoulis K.E., Trougakos I.P., & Papassideri I. (2011). Apolipoprotein J/Clusterin in Human Erythrocytes is Involved in the Molecular Process of Defected Material Disposal during Vesiculation. PLoS ONE 6, e26033.

Antonelou M.H., Kriebardis A.G., Stamoulis K.E., Trougakos I.P., & Papassideri I. (2011). Apolipoprotein J/Clusterin is a Novel Structural Component of Human Erythrocytes and a Biomarker of Cellular Stress and Senescence. PLoS ONE 6, e26032.

Leskov K.S., Araki S., Lavik J.P., Gomez J., Gama V., Gonos E.S., Trougakos I.P., Matsuyama S. & Boothman D.A. (2011). Crm1-Mediated Regulation Of Nuclear Clusterin (nCLU), An Ionizing Radiation-Stimulated, Bax-Dependent Pro-Death Factor. J. Biol. Chem. 286, 40083-40090.

Fandridis E., Apergis G., Korres D.S., Nikolopoulos K., Zoubos A.B., Papassideri I., & Trougakos I.P. (2011). Increased expression levels of Apolipoprotein J/Clusterin during primary osteoarthritis. In Vivo 25, 745-749.

Christodoulou A., Kostakis I.K., Kourafalos V., Pouli N., Marakos P., Trougakos I.P. & Tsitsilonis O.E. (2011). Design, Synthesis and Antiproliferative Activity of Novel Aminosubstituted Benzothiopyranoisoindoles. Bioorg. Med. Chem. Lett. 21, 3110-3112.


Trougakos I.P., Chondrogianni N., Amarantos I., Blake J., Schwager C., Ansorge W. & Gonos E.S. (2010). Genome-wide transcriptome profile of the human osteosarcoma Sa OS and U-2 OS cell lines. Cancer Genet. & Cytogenet. 196, 109-118.

Zhong B., Sallman D.A., Gilvary D.L., Pernazza D., Sahakian E., Fritz D., Cheng J.Q., Trougakos I., Wei S., & Djeu J.Y. (2010). Induction of Clusterin by AKT--Role in Cytoprotection against Docetaxel in Prostate Tumor Cells. Mol. Cancer Ther. 9, 1831-1841.


Trougakos I.P., Lourda M., Antonelou M.H., Kletsas D., Gorgoulis V.G., Papassideri I.S., Zou Y., Margaritis L.H., Boothman D.A. & Gonos E.S. (2009). Intracellular Clusterin inhibits mitochondrial apoptosis by suppressing p53-activating stress signals and stabilizing the cytosolic Ku70-Bax protein complex. Clinical Cancer Res. 15, 48-59. RESEARCH HIGHLIGHT

Balantinou E., Trougakos I.P., Chondrogianni N., Margaritis L.H. & Gonos E.S. (2009). Transcriptional and posttranslational regulation of clusterin by the two main cellular proteolytic pathways. Free Rad. Biol. Med. 46, 1267-1274.

Gonos E.S. Trougakos I.P., & Chondrogianni N. (2009). Aging Research in Greece. In: Palmore, E., Whitington, F. and Kunkel, S. (eds), pp. 249-257. International Handbook of Aging. Praeger Publishers, Connecticat, USA.

Markopoulou S., Kontargiris E., Batsi C., Tzavaras T., Trougakos I.P., Boothman D., Gonos E.S. & Kolettas E. (2009). Vanadium-induced apoptosis of HaCaT cells is mediated by c-fos and involves nuclear accumulation of clusterin. FEBS Journal 276, 3784-3799.

Trougakos I.P. & Gonos E.S. (2009). Oxidative stress in malignant progression: The role of Clusterin, a sensitive cellular biosensor of free radicals. Adv. Cancer Res. 104, 171-210.

Trougakos I.P., Djeu J.Y., Gonos E.S. & Boothman D.A. (2009). Advances and Challenges in Basic and Translational Research on Clusterin. Cancer Res. 69, 403-406.


Alexopoulos E.C., Cominos X., Trougakos I.P., Lourda M., Gonos E.S. & Makropoulos V. (2008). Biological monitoring of hexavalent chromium and serum levels of the senescence biomarker apolipoprotein j/clusterin in welders. Bioinorg Chem Appl. 2008:420578.

Chondrogianni N., Trougakos I.P., Kletsas D., Chen Q.M. & Gonos E.S. (2008). Partial proteasome inhibition in human fibroblasts triggers accelerated M1 senescence or M2 crisis depending on the p53 and Rb status. Aging Cell 7, 717-732. RESEARCH HIGHLIGHT

Lourda M., Trougakos I.P. & Gonos E.S. (2007). Development of resistance to chemotherapeutic drugs in human osteosarcoma cell lines largely depends on up-regulation of clusterin/apolipoprotein J. Int. J. Cancer 120, 611-622.

Papassideri I.S., Trougakos I.P., Leonard K.R. & Margaritis L.H. (2007). Crystalline yolk spheroids in Drosophila melanogaster oocyte: freeze fracture and two-dimensional reconstruction analysis. J. Insect Physiol. 53, 370-376.

Mocchegiani E., Giacconi R., Cipriano C., Costarelli L., Muti E., Tesei S., Giuli C., Papa R., Marcellini F., Mariani E., Rink L., Herbein G., Varin A., Fulop T., Monti D., Jajte J., Dedoussis G., Gonos E.S., Trougakos I.P. & Malavolta M. (2007). Zinc, metallothioneins, and longevity: effect of zinc supplementation: zincage study. Ann. N. Õ. Acad. Sci. 1119, 129-146.

Poulios E., Trougakos I.P., Chondrogianni N. & Gonos E.S. (2007). Exposure of human diploid fibroblasts to hypoxia extends proliferative life span. Ann. N. Y. Acad. Sci. 1119, 9-19.

Poulios E., Trougakos I.P. & Gonos E.S. (2006). Comparative effects of hypoxia on normal and immortalized human diploid fibroblasts. Anticancer Res. 26, 2165-2168.

Stratford F.L., Chondrogianni N., Trougakos I.P., Gonos E.S. & Rivett A.J. (2006). Proteasome response to interferon-ã is altered in senescent human fibroblasts. FEBS Lett. 580, 3989-3994.

Trougakos I.P., Pawelec G., Tzavelas C., Ntouroupi T. & Gonos E.S. (2006). Clusterin/Apolipoprotein J up-regulation after Zinc exposure, replicative senescence or differentiation of human haematopoietic cells. Biogerontology 7, 375-382.

Trougakos I.P., Petropoulou C., Franceschi C. & Gonos E.S. (2006). Reduced expression levels of the senescence biomarker Clusterin/Apolipoprotein J in lymphocytes from healthy centenarians. Ann. N. Y. Acad. Sci. 1067, 294-300.

Trougakos I.P., Saridaki A., Panayotou G. & Gonos E.S. (2006). Identification of differentially expressed proteins in senescent human embryonic fibroblasts. Mech. Ageing Dev. 127, 88-92.

Trougakos I.P. & Gonos E.S. (2006). Regulation of Clusterin/Apolipoprotein J, a functional homologue to the small heat shock proteins, by oxidative stress in ageing and age-related diseases. Free Radic. Res. 40, 1324-1334.

Psarra A-M.G., Solakidi S., Trougakos I.P., Margaritis L.H., Spyrou G. & Sekeris C.E. (2005). Glucocorticoid receptor isoforms in human hepatocarcinoma HepG2 and SaOS-2 osteosarcoma cells: Presence of glucocorticoid receptor alpha in mitochondria and of glucocorticoid receptor beta in nucleoli. Int. J. Biochem. Cell Biol. 37, 2544-2558.

Trougakos I.P., Lourda M., Agiostratidou G., Kletsas D. & Gonos E.S. (2005). Differential effects of Clusterin/Apolipoprotein J on cellular growth and survival. Free Rad. Biol. Med. 38, 436-449.

Katsiki M., Trougakos I.P., Chondrogianni N., Alexopoulos E.C., Makropoulos V. & Gonos E.S. (2004). Alterations of senescence biomarkers in human cells by exposure to CrVI in vivo and in vitro. Exp. Gerontol. 39, 1079-1087.

Kontargiris E., Kolettas E., Vadalouca A., Trougakos I.P., Gonos E.S. & Kalfakakou V. (2004). Ectopic expression of clusterin/apolipoprotein J or Bcl-2 decreases the sensitivity of HaCaT cells to toxic effects of ropivacaine. Cell Res. 14, 415-422.

Kouloukoussa M., Aleporou-Marinou V., Angelopoulou B., Trougakos I.P., Panagopoulou E., Kittas Chr. & Marinos E. (2004). Phorbol myristate acetate induces changes on F-actin and vinculin content in immature rat Sertoli cells. Tissue & Cell 36, 149-155.

Trougakos I.P. & Gonos E.S. (2004). Functional analysis of Clusterin/Apolipoprotein J in cellular death induced by severe genotoxic stress. Ann. N. Y. Acad. Sci. 1019, 206-210.

Trougakos I.P. So A., Jansen B., Gleave M.E. & Gonos E.S. (2004). Silencing expression of the Clusterin/Apolipoprotein J gene in human cancer cells using small interfering RNA induces spontaneous apoptosis, reduced growth ability and cell sensitization to genotoxic and oxidative stress. Cancer Res. 64, 1834-1842.

Chondrogianni N., Stratford F.L., Trougakos I.P., Friguet B., Rivett A.J. & Gonos E.S. (2003). Central role of the proteasome in senescence and survival of human fibroblasts: induction of a senescence-like phenotype upon its inhibition and resistance to stress upon its activation. J. Biol. Chem. 278, 28026-28037.

Papassideri I.S., Trougakos I.P., Leonard K.R. & Margaritis L.H. (2003). Structural and Biochemical Analysis of the Leptinotarsa decemlineata (Coleoptera: Chrysomeloidea) Crystalline Chorionic Layer. J. Insect Physiol. 49, 377-384.

Trougakos I.P., Poulakou M., Stathatos M., Chalikia Á., Melidonis A., & Gonos E.S. (2002). Serum levels of the senescence biomarker Clusterin/Apolipoprotein J increase significantly in diabetes type II and during development of coronary heart disease or at myocardial infarction. Exp. Gerontol. 37, 1175-1187.

Gonos E.S., Agrafiotis D., Dontas A.S., Efthimiopoulos S., Galaris D., Karamanos N.K., Kletsas D., Kolettas E., Panayotou G., Sekeri-Pataryas K.E., Simoes D., Sourlingas T.G., Stathakos D., Stratigos A.J., Tavernarakis N., Trougakos I.P. & Vynios D.H. (2002). Ageing research in Greece. Exp. Gerontol. 37, 735-747.

Trougakos I.P. & Gonos E.S. (2002). Clusterin/Apolipoprotein J in Human Aging and Cancer. Int. J. Biochem. Cell Biol. 34, 1430-1448.

Trougakos I.P., Chondrogianni N., Pimenidou A., Katsiki M., Tzavelas C. & Gonos E.S. (2002). Slowing down cellular aging in vitro. In: Rattan, S.I. (ed.): Modulating Aging and Longevity. Kluwer Academic Publishers, The Netherlands. Pp. 65-83.

Trougakos I.P. & Margaritis L.H. (2002). Novel Morphological and Physiological Aspects of Insect Eggs. In: Hilker, M. and Meiners, T. (eds.): Chemoecology of Insect Eggs and Egg Deposition. Blackwell Verlag GmbH, Berlin, Vienna. Pp. 3-36.

Petropoulou C., Trougakos I.P., Kolettas E., Toussaint O. & Gonos E.S. (2001). Clusterin/apolipoprotein J is a Novel Biomarker of Cellular Senescence, that does not affect the Proliferative Capacity of Human Diploid Fibroblasts. FEBS Lett. 509, 287-297.

Trougakos I.P., Papassideri I.S., Waring G.L. & Margaritis L.H. (2001). Differential Sorting of Constitutively co-secreted Proteins in the Ovarian Follicle Cells of Drosophila. Eur. J. Cell Biol. 80, 271-284. COVER PAGE

Kalantzi-Makri M.C., Trougakos I.P., Tafas T.P., Sourdis J. & Margaritis L.H. (1999). Phylogenetic and Taxonomical Relationships of the Eight Species in the melanogaster Subgroup of the Genus Drosophila (Sophophora) based on the Electrophoretic Mobility of the Major Chorion Proteins and the Eggshell Ultrastructure. J. Zool. 249, 295-306.

Trougakos I.P., Lamnissou K. & Margaritis L.H. (1999). Biochemical and Immunocytochemical Analysis of Vitellogenesis in the Olive Fruit Fly Dacus (Bactrocera) oleae (Diptera: Tephritidae). Cell Biol. Int. 23, 417-429.

Trougakos I.P. & Margaritis L.H. (1998a). The Formation of the Functional Chorion Structure of Drosophila virilis involves Intercalation of the “Middle” and “Late” Major Chorion proteins into a scaffold formed by the “Early” chorion proteins: A General Model for Chorion Assembly in Drosophilidae. J. Struct. Biol. 123, 97-110.

Trougakos I.P. & Margaritis L.H. (1998b). Immunolocalization of the Temporally “Early” Secreted Major Structural Chorion Proteins, Dvs38 and Dvs36, in the Eggshell Layers and Regions of Drosophila virilis. J. Struct. Biol. 123, 111-123.

Maniatis A.N., Trougakos I.P., Katsanis G., Palermos J., Maniatis N.A. & Legakis N.J. (1996). Changing Patterns of Bacterial Nosocomial Infections: A Nine-Year Study in a General Hospital. Chemotherapy 43, 69-76.



Gonos E.S. & Trougakos I.P. (2002). “A new method and kit for the quantitative measurement of the Clusterin/Apolipoprotein J levels in serum”. Hellenic Industrial Property Organization (No: 1004477, International itemization: G01N 33/68, C12Q 1/28).

Jansen B., Gleave M., Trougakos I.P., Gonos E.S., Signaevsky M. & Beraldi E. (2003). “RNAi probes targeting cancer-related proteins”. United States of America provisional application (No: 60/472,387), United States of America Patent application (No: 10/646,436).